Comparative transcriptional analysis of Bacillus subtilis cells overproducing either secreted proteins, lipoproteins or membrane proteins
نویسندگان
چکیده
منابع مشابه
Comparative transcriptional analysis of Bacillus subtilis cells overproducing either secreted proteins, lipoproteins or membrane proteins
BACKGROUND Bacillus subtilis is a favorable host for the production of industrially relevant proteins because of its capacity of secreting proteins into the medium to high levels, its GRAS (Generally Recognized As Safe) status, its genetic accessibility and its capacity to grow in large fermentations. However, production of heterologous proteins still faces limitations. RESULTS This study aim...
متن کاملProduction of recombinant proteins in Bacillus subtilis.
Project aims 1. Feasibility studies of Bacillus subtilis as an expression host for the production of therapeutically important proteins through high cell density fermentations. 2. Studies on intein mediated purification of therapeutically important proteins expressed in Bacillus subtilis. 3. Case studies with a few proteins of research/commercial relevance including malaria proteins that are li...
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In order to characterize the dynamics of the interaction between the emergent membrane translocated exoprotein and the components of Bacillus subtilis cell wall, we examined the kinetics of the in vitro refolding of levansucrase and alpha-amylase, at pH 7 and 37 degrees C, in the presence of polyphosphates (polyP) of various chain lengths (2</=n</=65). These soluble anionic polymers are conside...
متن کاملSeptal localization of forespore membrane proteins during engulfment in Bacillus subtilis.
In Bacillus subtilis, many membrane proteins localize to the sporulation septum, where they play key roles in spore morphogenesis and cell-specific gene expression, but the mechanism for septal targeting is not well understood. SpoIIQ, a forespore-expressed protein, is involved in engulfment and forespore-specific gene expression. We find that SpoIIQ dynamically localizes to the sporulation sep...
متن کاملTowards the development of Bacillus subtilis as a cell factory for membrane proteins and protein complexes
BACKGROUND The Gram-positive bacterium Bacillus subtilis is an important producer of high quality industrial enzymes and a few eukaryotic proteins. Most of these proteins are secreted into the growth medium, but successful examples of cytoplasmic protein production are also known. Therefore, one may anticipate that the high protein production potential of B. subtilis can be exploited for protei...
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ژورنال
عنوان ژورنال: Microbial Cell Factories
سال: 2012
ISSN: 1475-2859
DOI: 10.1186/1475-2859-11-66